|
Congo Red Stain Kit
Кит за оцветяване Congo Red
|
This kit is used to detect Amyloid Protein in tissue sections using Congo Red, a metachromatic stain.
The staining should be intensified by pretreatment with an Alkaline Sodium Chloride Solution.
 Principle
This is an empirical method. It is still doubtful whether the formation of the link between amyloid and dye is due to proteins or to glycans in amyloid, or even to both. On the contrary, it is certain that amyloid stained with Congo red displays a marked birefringence in polarized light. From this anisotropy it has been inferred that amyloid may have an intrinsic molecular organization which is emphasized by Congo red. According to Wirchow and Puchtler, amlyloid is the only component in human tissues whose histochemical characteristics can be compared to those of cellulose; therefore it has been supposed that dye and amyloid are linked by hydrogen bonds, just as it happens with the link between cellulose and Congo red.
Contents:
Congo Red Solution 250ml
Alkaline Sodium Chloride Solution 250ml
Haemalum Mayer 250ml
Sodium Hydroxide Solution 12m |
|
|
|
